Gel electrophoresis

From MIBBI

1. General features

1.1. Date

1.2. Person or responsible role

1.3. Electrophoresis type

2. Sample

2.1. Sample name(s)

2.2. Loading buffer

3. Gel matrix and electrophoresis¹

3.1. Dimension details

3.1.1. Ordinal number for this dimension

3.1.2. Separation method employed

3.2. Gel matrix

3.2.1. Description of gel matrix

3.2.2. Gel manufacturer

3.2.3. Physical dimensions

3.2.4. The physicochemical property range and distribution (as appropriate)

3.2.5. Acrylamide concentration

3.2.6. Acrylamide to crosslinker ratio

3.2.7. Additional substances in gel

3.2.8. Gel lane

3.2.9. Sample application

3.3. Protocol

3.3.1. Buffers

3.3.2. Electrophoresis conditions

4. Inter-dimension process²

4.1. Inter-dimension process

4.1.1. Step name

4.1.2. Inter-dimension buffer

4.1.3. Additional reagents

4.1.4. Equipment

4.1.5. Protocol

5. Detection

5.1. Direct detection

5.1.1. Name of direct detection process

5.1.2. Direct detection agents

5.1.3. Additional reagents and buffers

5.1.4. Equipment

5.1.5. Direct detection protocol

5.2. Indirect detection

5.2.1. Name of indirect detection process

5.2.2. Transfer medium

5.2.3. Detection medium

5.2.4. Indirect detection agents

5.2.5. Additional reagents and buffers

5.2.6. Equipment

5.2.7. Indirect detection protocol

6. Image acquisition³

6.1. Acquisition equipment

6.1.1. Type of equipment

6.1.2. Name of equipment

6.1.3. Software

6.1.4. Calibration (if appropriate)

6.1.5. Equipment specific parameters

6.2. Acquisition protocol

6.2.1. Image acquisition process

6.2.2. Reference to gel matrix

7. Image⁴

7.1. Image name or ID

7.2. Dimensions

7.3. Resolution

7.4. Bit depth

7.5. Image location

7.6. Standard image orientation

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Footnotes

¹ There should be one description for each dimension. Each dimension must have a description for Dimension details, Gel matrix and Protocol. If a gel is composed from two or more matrices (e.g. stacking gel and resolving gel), each matrix must have a Gel matrix description.

² This section is used to record any process or processes applied to, or carried out between the dimensions described in Gel matrix and electrophoresis. Not applicable for one-dimensional gel electrophoresis.

³ If applicable

⁴ The result of a previously-described Image acquisition.

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Sources

MIAPE

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Notes

This module identifies the minimum information required to report the use of gel electrophoresis in a proteomics experiment, sufficient to support both the effective interpretation and assessment of the data and the potential recreation of the work that generated it.