Nucleic acid sequencing data processing
From MIBBI
- 1. Chimera check1
- 2. Assembly
- 2.1. Assembly method2
- 2.2. Estimated error rate3
- 2.3. Method of calculation of error rate
- 3. Finishing strategy
- 3.1. Status4
- 3.2. Coverage5
- 3.3. Number of contigs6
- 4. Additional information
- 4.1. Relevant Standard Operating Procedures (SOPs)
- 4.2. Relevant electronic resources
Footnotes
- 1 State whether the data have been checked for chimeric sequence, and how.
- 2 How was the assembly done (e.g. with a text based assembler like phrap or a flowgram assembler).
- 3 The estimated error rate associated with the finished sequences (e.g., error rate of 1 in 1000 bp).
- 4 Was the genome project intended to produce a complete or draft genome.
- 5 The fold coverage of the sequencing expressed as 2x, 3x, 18x etc.
- 6 Number of contigs produced for the genome.
Sources
- MIGS
Notes
